Category Bacteriology

Laboratory Diagnosis of Gonorrhea

The name gonorrhea, which means “flow of seed,” was initially used in the second century when the urethral discharge was mistaken for sperm. For years, the illnesses syphilis and gonorrhea were confused since they were frequently seen in the same…

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LAP (Leucine Aminopeptidase) Test

Leucine aminopeptidase (LAP) is an enzyme that is employed in a test for the presumptive identification of Streptococci. LAP is a peptidase that hydrolyzes peptide bonds near free amino groups. LAP is known as leucine aminopeptidase because it responds most…

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Mycoplasma

Because of their small size and lack of a cell wall, mycoplasmas vary from other bacteria. Mycoplasmas are distinguishable from other bacteria in the Mollicutes (mollis, soft; cutis, skin, in Latin) class by their lack of cell walls in taxonomy.…

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Triple Sugar Iron Agar

Triple Sugar Iron (TSI) Agar and iron Agar (KIA) from Kligler are useful in the presumptive identification of gram-negative enteric bacteria, especially in the screening for intestinal pathogens. The formulae for TSI agar and KIA are identical except that in…

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Antimicrobial mechanism of action

Introduction Clinically effective antimicrobial agents exhibit selective toxicity towards the microbe rather than the host, a feature which distinguishes them from the disinfectants. Selectivity is described in most cases by action on microbial processes or structures which differ from those…

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Catalase Test

PRINCIPLE Bacteria that produce the enzyme catalase break down hydrogen peroxide into water and gaseous oxygen, allowing gas bubbles to escape. Most bacteria can be identified using this test. REAGENTS AND SUPPLIES A.Hydrogen peroxide reagent 30% For Neisseria30 percent H2O2…

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