Molecular Diagnostics

How does magnetic bead DNA extraction work?

How does magnetic bead DNA extraction work?

Separating nucleic acid is an exceedingly essential tool for molecular biology. Until modern technology could be used, nucleic acid separation was a time-consuming and work-intensive process based on multiple extraction and centrifugation steps, often constrained by small yields and low purity of separation products, and not suitable for automation and upscaling. Specifically functionalized magnetic particles…

Minimum Information Necessary for Quantitative Real-Time PCR Experiments

Minimum Information Necessary for Quantitative Real-Time PCR Experiments

The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines reflect a significant milestone for the transition of a robust “gold standard” in the process of turning the accurate quantitative polymerase chain reaction (qPCR) from a testing technique into real-time quantitative polymerase reaction (QPCR). It has allowed legacy PCRs to easily be transferred…

Real-Time PCR

Real-Time PCR

Basics of PCR Real-time PCR is a continuous collection of fluorescent signals from one or more reactions in the polymerase chain over a number of cycles. Quantitative real-time PCR is the processing for each sample of the fluorescent signals from each reaction into a number value. Normally, PCR is focused on the quantitative relationship between…

DNA sequencing technologies

DNA sequencing technologies

The advent of sequencing techniques has played an important role in the study of organism genomic sequences. Tedious were early attempts to sequence DNA; 12 bases of coherent ends of bacteriophage were calculated through the usage of key extension methods. Maxam and Gilbert identified 24 bases of the binding site for lactose-repressors by copying them…