How does magnetic bead DNA extraction work?

Separating nucleic acid is an exceedingly essential tool for molecular biology. Until modern technology could be used, nucleic acid separation was a time-consuming and work-intensive process based on multiple extraction and centrifugation steps, often constrained by small yields and low purity of separation products, and not suitable for automation and …

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Interpreting Real-Time PCR Amplification Plot

Proper data analysis is crucial to obtaining valid and relevant results from an experimental system but is especially critical when assessing variations in mRNA expression of genes, the so-called transcriptome. Unlike DNA that is present in each cell throughout the life of the organism, RNA is transiently expressed and levels …

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Minimum Information Necessary for Quantitative Real-Time PCR Experiments

The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines reflect a significant milestone for the transition of a robust “gold standard” in the process of turning the accurate quantitative polymerase chain reaction (qPCR) from a testing technique into real-time quantitative polymerase reaction (QPCR). It has allowed legacy …

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DNA sequencing technologies

The development of sequencing technology has contributed to the study of organisms’ genomic sequences. Maxam and Gilbert found the binding site for lactose-repressors by copying and sequencing 24 bases. Sanger and Maxam-Gilbert procedures were more widespread previously and are still used to determine the order of the nucleotide bases adenine, …

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