biochemical test Archives » Clinical Laboratory Science

Triple Sugar Iron Agar

Triple Sugar Iron (TSI) Agar and iron Agar (KIA) from Kligler are useful in the presumptive identification of gram-negative enteric bacteria, especially in the screening for intestinal pathogens. The formulae for TSI agar and KIA are identical except that in addition to glucose and lactose, TSI agar contains sucrose. TSI …

Catalase Test

by clinicalsci

PRINCIPLE Bacteria that produce the enzyme catalase break down hydrogen peroxide into water and gaseous oxygen, allowing gas bubbles to escape. Most bacteria can be identified using this test. REAGENTS AND SUPPLIES A. Hydrogen peroxide reagent 30% For NeisseriaCaution is needed since 30% H2O2 is very corrosive to the skin. …