Theory & Practices of Clinical Laboratory
Quantitative real-time PCR (qPCR) has become a new normal in molecular biology for evaluating the gene expression. This technique allows for sensitive and specific detection of mRNA levels, forming the basis of transcriptomic studies. However, because RNA is dynamic and transient, especially when compared to the more stable DNA, optimizing …
The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines reflect a significant milestone for the transition of a robust “gold standard” in the process of turning the accurate quantitative polymerase chain reaction (qPCR) from a testing technique into real-time quantitative polymerase reaction (QPCR). It has allowed legacy …
Basics of PCR During a number of amplification cycles, real-time PCR collects fluorescent signals from reactions in the polymerase chain. Quantitative real-time PCR involves converting the fluorescent signals from reactions into a numerical value for each sample. PCR is normally based on a quantitative relationship between the quantities of target …