qPCR

Minimum Information Necessary for Quantitative Real-Time PCR Experiments

Minimum Information Necessary for Quantitative Real-Time PCR Experiments

The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines reflect a significant milestone for the transition of a robust “gold standard” in the process of turning the accurate quantitative polymerase chain reaction (qPCR) from a testing technique into real-time quantitative polymerase reaction (QPCR). It has allowed legacy PCRs to easily be transferred…

Real-Time PCR

Real-Time PCR

Basics of PCR Real-time PCR is a continuous collection of fluorescent signals from one or more reactions in the polymerase chain over a number of cycles. Quantitative real-time PCR is the processing for each sample of the fluorescent signals from each reaction into a number value. Normally, PCR is focused on the quantitative relationship between…