Theory & Practices of Clinical Laboratory
Proper data analysis is crucial to obtaining valid and relevant results from an experimental system but is especially critical when assessing variations in mRNA expression of genes, the so-called transcriptome. Unlike DNA that is present in each cell throughout the life of the organism, RNA is transiently expressed and levels …
The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines reflect a significant milestone for the transition of a robust “gold standard” in the process of turning the accurate quantitative polymerase chain reaction (qPCR) from a testing technique into real-time quantitative polymerase reaction (QPCR). It has allowed legacy …
Basics of PCR During a number of amplification cycles, real-time PCR collects fluorescent signals from reactions in the polymerase chain. Quantitative real-time PCR involves converting the fluorescent signals from reactions into a numerical value for each sample. PCR is normally based on a quantitative relationship between the quantities of target …